Original Articles

Takemori A, Kaulich PT, Cassidy L, Takemori N, Tholey A. Size-Based Proteome Fractionation through Polyacrylamide Gel Electrophoresis Combined with LC-FAIMS-MS for In-Depth Top-Down Proteomics. Anal Chem. 2022 Sep 20;94(37):12815-12821. doi: 10.1021/acs.analchem.2c02777.


Takemori A, Kawashima Y, Takemori N. Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column. Chem Commun (Camb). 2021 Dec 12. doi: 10.1039/d1cc05529a.  (Selected for ChemComm's Inside Front Cover Art)


Valgepea K, Talbo G, Takemori N, Takemori A, Ludwig C, Mahamkali V, Mueller AP, Tappel R, Köpke M, Simpson SD, Nielsen LK, Marcellin E. Absolute Proteome Quantification in the Gas-Fermenting Acetogen Clostridium autoethanogenum. mSystems. 2022 Apr 26;7(2):e0002622. 


Johnson J, Harman VM, Franco C, Emmott E, Rockliffe N, Sun Y, Liu LN, Takemori A, Takemori N, Beynon RJ. Construction of à la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins. BMC Biol. 2021 Sep 8;19(1):195. doi: 10.1186/s12915-021-01135-9.


Endo Y, Takemori N, Nagy SK, Okimune KI, Kamakaka R, Onouchi H, Takasuka TE. De novo reconstitution of chromatin using wheat germ cell-free protein synthesis. FEBS Open Bio. 2021 Jun;11(6):1552-1564. doi: 10.1002/2211-5463.13178. 


Ishizaki J, Takemori A, Horie K, Hiraoka D, Suemori K, Matsumoto T, Sada KE, Amano K, Harigai M, Arimura Y, Makino H, Takenaka K, Takemori N, Hasegawa H; Research Committee of Intractable Vasculitis Syndrome and the Research Committee of Intractable Renal Disease of the Ministry of Health, Labour and Welfare of Japan. Usefulness of tissue inhibitor of metalloproteinase 1 as a predictor of sustained remission in patients with antineutrophil cytoplasmic antibody-associated vasculitis. Arthritis Res Ther. 2021 Mar 20;23(1):91. doi: 10.1186/s13075-021-02471-5.


Takemori A, Ishizaki J, Nakashima K, Shibata T, Kato H, Kodera Y, Suzuki T, Hasegawa H, Takemori N. BAC-DROP: Rapid Digestion of Proteome Fractionated via Dissolvable Polyacrylamide Gel Electrophoresis and Its Application to Bottom-Up Proteomics Workflow. J Proteome Res. 2021 Mar 5;20(3):1535-1543. doi: 10.1021/acs.jproteome.0c00749. Epub 2020 Dec 24. (Selected for JPR's Supplmentary Cover Art)


Takemori A, Butcher DS, Harman VM, Brownridge P, Shima K, Higo D, Ishizaki J, Hasegawa H, Suzuki J, Yamashita M, Loo JA, Loo RRO, Beynon RJ, Anderson LC, Takemori N. PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry. J Proteome Res. 2020 Jul 11. doi: 10.1021/acs.jproteome.0c00303.

(Selected for ACS Editor's Choice and JPR's Supplmentary Cover Art)


Toriyama K, Kuwahara M, Kondoh H, Mikawa T, Takemori N, Konishi A, Yorozuya T, Yamada T, Soga T, Shiraishi A, Yamashita M. T cell-specific deletion of Pgam1 reveals a critical role for glycolysis in T cell responses. Commun Biol. 2020 Jul 24;3(1):394. doi: 10.1038/s42003-020-01122-w.


Takemori A, Nakashima T, Ômura H, Tanaka Y, Nakata K, Nonami H, Takemori N. Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy. Plant Methods. 2019 Apr 24;15:40. doi: 10.1186/s13007-019-0427-7.


Valgepea K, de Souza Pinto Lemgruber R, Abdalla T, Binos S, Takemori N, Takemori A, Tanaka Y, Tappel R, Köpke M, Simpson SD, Nielsen LK, Marcellin E. H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum. Biotechnol Biofuels. 2018 Mar 1;11:55. doi: 10.1186/s13068-018-1052-9.


Takemori N, Takemori A, Tanaka Y, Endo Y, Hurst JL, Gómez-Baena G, Harman VM, Beynon RJ. MEERCAT: MULTIPLEXED EFFICIENT CELL FREE EXPRESSION OF RECOMBINANT QconCATS FOR LARGE SCALE ABSOLUTE PROTEOME QUANTIFICATION. Mol Cell Proteomics. 2017 Oct 20. pii: mcp.RA117.000284. doi: 10.1074/mcp.RA117.000284.(Selected for MCP's Cover Art)


Ishizaki J, Takemori A, Suemori K, Matsumoto T, Akita Y, Sada KE, Yuzawa Y, Amano K, Takasaki Y, Harigai M, Arimura Y, Makino H, Yasukawa M, Takemori N, Hasegawa H; Research Committee of Intractable Vasculitis Syndrome and the Research Committee of Intractable Renal Disease of the Ministry of Health, Labour and Welfare of Japan. Targeted proteomics reveals promising biomarkers of disease activity and organ involvement in antineutrophil cytoplasmic antibody-associated vasculitis. Arthritis Res Ther. 19(1):218, 2017


Takemori N, Takemori A, Wongkongkathep P, Nshanian M, Loo RRO, Lermyte F, Loo JA. Top-down/Bottom-up Mass Spectrometry Workflow Using Dissolvable Polyacrylamide Gels. Anal Chem. 89:8244-8250, 2017


Kuwahara M, Ise W, Ochi M, Suzuki J, Kometani K, Maruyama S, Izumoto M, Matsumoto A, Takemori N, Takemori A, Shinoda K, Nakayama T, Ohara O, Yasukawa M, Sawasaki T, Kurosaki T, Yamashita M. Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat Commun. 7:12596, 2016


Takemori N, Takemori A, Tanaka Y, Ishizaki J, Hasegawa H, Shiraishi A, Ohashi Y. High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system. Mol Biosyst. 12:2389-2393, 2016


Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M, Takeda H, Sawasaki T, Endo Y, and Higashiyama S. High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system. Mol Biosyst.11:361-365, 2015


Nemoto K, Takemori N, Seki M, Shinozaki K, Sawasaki T. Members of the Plant CRK-superfamily are Capable of trans-/auto-Phosphorylation of Tyrosine Residues. J Biol Chem. 2015 May 12. pii: jbc.M114.617274.  


Yokoi S, Okuyama T, Kamei Y, Naruse K, Taniguchi Y, Ansai S, Kinoshita M, Young LJ, Takemori N, Kubo T, and Takeuchi H. An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of medaka fish (Oryzias latipes). PLoS Genet. 11(2):e1005009, 2015


Takemori N, Takemori A, Ishizaki J, and Hasegawa H.  Enzymatic protein digestion using a dissolvable polyacrylamide gel and its application to mass spectrometry-based proteomics. J Chromatogr B Analyt Technol Biomed Life Sci. 967:36-40, 2014


Shirato A, Kikugawa T, Miura N, Tanji N, Takemori N, Higashiyama S, and Yokoyama M.  Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells. Oncology Letters. 7:674-678, 2014


Miwa D, Sakaue T, Inoue H, Takemori N, Kurokawa M, Fukuda S, Omi K, Goishi K, and Higashiyama S.  Protein kinase D2 and heat shock protein 90 beta are required for BCL6-associated zinc finger protein mRNA stabilization induced by vascular endothelial growth factor-A. Angiogenesis. 16:675-688 2013


Miura N, Takemori N, Kikugawa T, Tanji N, Higashiyama S, and Yokoyama M.  Adseverin: A novel cisplatin-resistant marker in the human bladder cancer cell line HT1376 identified by quantitative proteomic analysis. Molecular Oncology. 6:311-322, 2012


Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, Miwa D, Nishiwaki E, Hatano M, Tokuhisa T, Endo Y, Nose M, and Higashiyama S.  BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signaling in angiogenesis.  Blood. 119:2688-2698, 2012


Matsumoto H, Takemori N, and Komori N.  Methods in Molecular Biology, Protein identification on archived 2-D gels, 869:305-308, 2012


Mecklenburg KL, Takemori N, Komori N, Chu B, Hardie RC, Matsumoto H, and O'Tousa JE.  Retinophilin is a light-regulated phosphoprotein required to suppress photoreceptor dark noise in Drosophila. The Journal of Neuroscience. 30:1238-1249, 2010 


Takemori N and Yamamoto MT.  Proteome mapping of the Drosophila melanogaster male reproductive system. Proteomics. 9:2484-2493, 2009 (Selected for Faculty of 1000 Biology)


Shitama T, Hayashi H, Noge S, Uchio E, Oshima K, Haniu H, Takemori N, Komori N, and Matsumoto H. Proteome profiling of vitreoretinal diseases by cluster analysis. Proteomics Clinical Applications. 2:1265-1280, 2008 


Elliott MH, Nash ZA, Takemori N, Fliesler SJ, McClellan ME, and Naash MI.  Differential distribution of proteins and lipids in detergent-resistant and detergent-soluble domains in rod outer segment plasma membranes and disks. Journal of Neurochemistry. 104:336-352, 2008 


Tsuji T, Hirota T, Takemori N, Komori N, Yoshitane H, Fukuda M, Matsumoto H, and Fukada Y. Circadian proteomics of the mouse retina. Proteomics. 7:3500-3508, 2007


Takemori N, Komori N, Thompson JN Jr, Yamamoto MT, and Matsumoto H.  Novel eye-specific calmodulin methylation characterized by protein mapping in Drosophila melanogaster.  Proteomics. 7: 2651-2658, 2007 (Selected for Wiley PROTEOMICS cover illustration)


Komori N, Takemori N, Kim HK, Singh A, Hwang SH, Foreman RD, Chung K, Chung JM, and Matsumoto H.  Proteomics study of neuropathic and nonneuropathic dorsal root ganglia: altered protein regulation following segmental spinal nerve ligation injury.  Physiological Genomics. 29:215-230, 2007


Hurst RE, Kyker KD, Dozmorov MG, Takemori N, Singh A, Matsumoto H, Saban R, Betgovargez E, and Simonian MH.  Proteome-level display by 2-dimensional chromatography of extracellular matrix-dependent modulation of the phenotype of bladder cancer cells.  Proteome Science. 4:1-11, 2006


Haniu H, Komori N, Takemori N, Singh A, Ash JD, and Matsumoto H.  Proteomic trajectory mapping of biological transformation: application to developmental mouse retina. Proteomics. 6:3251-3261, 2006


Takemori N, Komori N, and Matsumoto H.  Highly sensitive multistage mass spectrometry enables small-scale analysis of protein glycosylation from two-dimensional polyacrylamide gels. Electrophoresis. 27:1394-1406, 2006 (Selected for Proteomics Key Papers of Wiley Electrophoresis 2006)




Takemori N, Takemori A

In-depth structural proteomics integrating mass spectrometry and polyacrylamide gel electrophoresis

Frontiers in Analytical Science 2023, Vol.2




電気泳動 2022年66巻pp.53-57




電気泳動 2021年65巻2号pp.63-68




炎症と免疫 2020年7月号 (Vol.28 No.4)




炎症と免疫 2020年1月号 (Vol.28 No.1)


Takemori N.

A multiplexed co-synthesis system for stable isotope-labeled peptide standards using wheat germ cell-free synthesis and its application to quantitative proteomics. Proteome Letters, 2018, Vol.3, pp.23-30 (Japanese)


Takemori N.

High-throughput quantitative analysis of transmembrane proteins using mass spectrometry. Biophysics, 2016, 56, 116 (Japanese)


Takemori N.

Targeted protein quantitation using selected reaction monitoring. Seikagaku. 2015 87:636-641. (Japanese)


Yamamoto MT and Takemori N.  Proteome profiling reveals tissue-specific protein expression in the male reproductive system of Drosophila melanogaster.  Fly (Austin), 4:36-39, 2010 


Matsumoto H, Takemori N, Thompson JN Jr, Yamamoto MT, and Komori N.  Drosophila Proteome Atlas.  Drosophila Information Service, 90:162-164, 2007


武森信暁,小森直香,松本博行 2次元ゲル電気泳動と質量分析を用いた蛋白質のリン酸化および糖鎖修飾の微量解析. 蛋白質核酸酵素, 52:456-462, 2007


Book Chapters

武森信曉 CSJ Current Review, Vol. 45, 生体分子環境の化学 : 分子夾雑と1分子で解き明かす生体の挙動 

日本化学会 (担当:分担執筆, 範囲:14章 分子夾雑環境におけるプロテオフォーム解析のための質量分析)

化学同人 2023


Takemori A, Takemori N. Integrated Methods in Protein Biochemistry: Part C, Chapter Six - Sample preparation for structural mass spectrometry via polyacrylamide gel electrophoresis. Methods Enzymol. 2023;682:187-210. doi: 10.1016/bs.mie.2022.08.051.


武森信暁,山本雅敏  モデル動物利用マニュアル:生物機能モデルと新しいリソース・リサーチツール,第3節 哺乳類以外のモデル動物  第1項 ショウジョウバエ (5) 質量分析法によるショウジョウバエ組織の分子プロファイリング, 552-556, 株式会社エル・アイ・シー, 2011


Takemori N, Komori N, and Matsumoto H.  The Protein Protocols Handbook Third edition, Part V (150): Post-translational modifications. MS analysis of protein glycosylation, 1387-1394, Springer, 2009